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Expression and Purification of Human Lysosomal β-galactosidase from Pichia Pastoris
human β-galactosidase lysosomal storage diseases Pichia pastoris protein expression protein purification biochemistry and molecular biology
2014/12/12
Lysosomal storage diseases are genetically inherited diseases caused by the dysfunction of lysosomal enzymes. In a normal cell, lysosomal enzymes cleave specific macromolecules as they are transported...
Structural And Biochemical Studies Of The Human Lysosomal Enzymes: N-Acetylgalactosamine-6-Sulfatase, N-Sulfoglucosamine Sulfohydrolase And Beta-Galactosidase
Pure sciences Biological sciences Cysteine modification Enzyme replacement therapy Lysosomal storage disease Pharmacological chaperone therapy Sulfatase structure X-ray crystallography
2014/12/10
Lysosomal storage diseases are disorders caused by deficiencies of enzymes responsible for the degradation of substances present in lysosomes. The loss of activity of a lysosomal enzyme leads to the a...
Improving Soluble Expression of β-Galactosidase in Escherichia coli by Fusion with Thioredoxin
Galactosidase Thioredoxin Inclusion Body IPTG
2016/4/18
Recombinant heterologous proteins can be produced as insoluble aggregates partially or perfectly inactive in Escherichia coli. One of the strateges to improve the solubility of recombinant proteins is...
β-Galactosidase Gene of Thermus thermophilus KNOUC112 Isolated from Hot Springs of a Volcanic Area in New Zealand: Identification of the Bacteria, Cloning and Expression of the Gene in Escherichia coli
Thermus thermophilus Cloning and Expression
2016/4/18
To isolate the β-galactosidase producing thermophilic bacteria, samples of mud and water were collected from hot springs of avolcanic area near Golden Springs in New Zealand. Among eleven isolated str...
In Vitro Stability of β-galactosidase Microcapsules
In vitro stability Microcapsule Lactase Milk
2016/4/12
The present study was carried out to examine the efficiency of microcapsules and a stability of lactase in vitro in the simulated gastric and intestinal conditions. As a coating materials, medium-chai...
Treatment of Microencapsulated β- Galactosidase with Ozone : Effect on Enzyme and Microorganism
Ozone Treatment Microencapsulation Lactase Milk
2016/4/8
The present study was designed to examine the effect of ozone treatment in microencapsulated ß-galactosidase on inactivation of the enzyme and sterilization of microorganism. The efficiency was t...