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Characterization of boar sperm plasma membranes by two-dimensional PAGE and isolation of specific groups of polypeptides by anion exchange chromatography and lectin affinity chromatography
plasma membranes spermatozoa twodimensional
2009/2/19
High resolution, two-dimensional PAGE was used to characterize purified plasma membranes (PM) from boar spermatozoa. In addition to the abundance of polypeptides resolved, unusual features of PM dis...
Electrophoretic and Chromatographic Properties of Boar Sperm Plasma Membranes：Antigens and Polypeptides with Affinity for Isolated Zonae Pellucidae
sperm plasma membrane zonae pellucidae
2009/2/19
Two methods were used to assess the ability of plasma membrane (PM) polypeptides to bind to isolated zonae pellucidae (ZP). Crossed immunoelectrophoresis patterns of detergent solubilized PM were co...
Glycosaminoglycan Stimulation of the In Vitro Conversion of Boar Proacrosin into Acrosin
acrosomal enzymes acrosome reaction proacrosin acrosin glycosaminoglycan
2009/2/18
Glycosaminoglycans obtained from shark cartilage, whale cartilage, porcine intestinal mucosa, porcine skin, and human umbilical cord were found to accelerate the in vitro conversion of highly puri...
Exposure of human, boar, or bull sperm to a synthetic peptide increases binding to an egg-membrane substrate
human boar bull sperm synthetic peptide increases egg-membrane substrate
2009/2/18
We evaluated the effects of in vitro exposure of sperm to synthetic FertPlus peptide, which represents a 60-amino acid sequence within rat prosaposin, using a microwell sperm-binding assay (SBA), ...
Extender components and surfactants affect boar sperm function and membrane behavior during cryopreservation
Extender components surfactants boar sperm function membrane behavior cryopreservation
2009/2/18
To determine how the individual components of extenders affected boar sperm function and membrane structure and to test a new surfactant's cryoprotective ability, boar sperm were cryopreserved in ...
Objectively measured boar sperm motility parameters correlate with the outcomes of on-farm inseminations：results of two fertility trials
Semen analysis CASA spermatozoa
2009/2/17
Two fertility trials were undertaken to evaluate the relationship between boar semen quality and fertility (conception rate and litter size) after on-farm artificial insemination (AI). Trial 1 inclu...
Choice of operating conditions to minimize sperm subpopulation sampling bias in the assessment of boar semen by computer-assisted semen analysis
CASA computer semen analysis tracking semen
2009/2/16
The performance of a computer-assisted semen analysis system was evaluated for use with washed boar spermatozoa. Accuracy was tested using a computer graphics-generated series of spots moving along ...
Comparative study of boar sperm coming from the caput, corpus, and cauda regions of the epididymis
boar sperm caput corpus cauda regions epididymis
2009/2/13
Sperm quality in the caput, corpus, and cauda regions of the epididymis of healthy and sexually mature Landrace boars was studied. Epididymal sperm characteristics were examined by light microscopy (L...
Movement characteristics of boar sperm obtained from the oviduct or hyperactivated in vitro
Pig uterine tube fallopian tube sperm motility
2009/2/10
The objectives of this study were to describe hyperactivated motility in boar sperm and to determine the incidence of hyperactivation among boar sperm flushed from the oviduct. Oviducts were surgica...
Cryopreservation in different concentrations of glycerol alters boar sperm and their membranes
Swine sperm motility acrosome membrane fluidity
2008/12/29
To test the hypothesis that glycerol would concomitantly affect sperm membrane structure and the function of the intact cells, boar semen (4 ejaculates from 4 boars) was cryopreserved in an egg yo...
Morphologically distinct sperm subpopulations defined by Fourier shape descriptors in fresh ejaculates correlate with variation in boar semen quality following cryopreservation
sperm subpopulations Fourier shape descriptors fresh ejaculates boar semen quality
2008/12/26
This study investigated two hypotheses: 1) that consistent between-boar variation in frozen semen quality exists and is genetically determined, and 2) that morphologically distinct subpopulations...
Effects of methyl-beta-cyclodextrin on cryosurvival of boar spermatozoa
methyl-beta-cyclodextrin cryosurvival boar spermatozoa
2008/12/26
Methyl-beta-cyclodextrin (MBCD), which leads to the stimulation of cholesterol efflux from the cell membrane, was examined for its ability to increase the cryosurvival of spermatozoa from Gottingen mi...
Effects of Seminal Plasma on Cooling-Induced Capacitative Changes in Boar Sperm
Cryopreservation semen capacitation
2008/12/24
Porcine seminal plasma (SP) has been shown to contain factors that have a decapacitative or capacitation-inhibiting effect on sperm. The objectives of the present study were to compare the capaci...
Sperm Sorting Procedure Induces a Redistribution of Hsp70 but Not Hsp60 and Hsp90 in Boar Spermatozoa
Flow cytometry heat shock protein 60 heat shock protein 70 heat shock protein 90 pig
2008/12/24
Heat shock proteins, besides their protective function against stresses, have been recently indicated as key factors for sperm fertilizing ability. Since sexing sperm by high-speed flow-cytometry su...
Immunolocalization and Possible Functional Role of PSP-I/PSP-II Heterodimer in Highly Extended Boar Spermatozoa
Reproductive tract semen sperm seminal plasma sperm capacitation
2008/12/24
PSP-I/PSP-II heterodimer is a major protein of boar seminal plasma which is able to preserve, in vitro, the viability, motility, and mitochondrial activity of highly extended boar spermatozoa for at...