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Cloning of the Recombinant Cytochrome P450 Cyp141 Protein of Mycobacterium tuberculosis as a Diagnostic Target and Vaccine Candidate
Cloning Cytochrome Mycobacterium Tuberculosis Diagnostic
2015/10/15
Background: Tuberculosis has been announced as a global emergency by World Health Organization and the second infectious agent of mortality worldwide. The general policy in the development of new vacc...
Cloning and Sequence Analysis of LipL32, a Surface–Exposed Lipoprotein of Pathogenic Leptospira Spp
Leptospirosis Leptospira Interrogans LipL32
2015/10/9
Background: Leptospirosis is a worldwide zoonosis caused by pathogenic Leptospira species. A major challenge of this disease is the application of basic research to improve diagnostic methods and rela...
Cloning, Expression, Characterization, and Computational Approach for Cross-Reactivity Prediction of Manganese Superoxide Dismutase Allergen from Pistachio Nut
cloning cross-reaction Manganese Superoxide Dismutase (MnSOD) pistachio (Pistacia vera)
2010/11/12
The MnSOD gene from pistachio was successfully cloned and expressed in E. coli. The purified pistachio MnSOD was recognized by IgE in 10 (40%) out of the 25 sera tested. Our results also showed that t...
Background: Human embryonic stem (hES) cells have the unrivaled ability to differentiate into any specialized cell type. Significant attention is currently directed to the biological and therapeutic c...
Isolation,Cloning,Expression and Purification of Recombinant RhD Antigen from Cord Blood
Recombinant RhD antigen Cloning Cord blood
2009/12/7
Background: Rh (Rhesus) is a highly complex blood group system in man deeply rooted in transfusion medicine. Isolation of RhD from cord blod, cloning and expression of recombinant RhD antigen in bacte...
Cloning and Expression of the Allergen Cro s 2 Profilin from Saffron (Crocus sativus)
allergy cloning Cro s 2 profilin recombinant allergen saffron
2010/11/16
Background: Profilin is a panallergen that is recognized by IgE in allergic patients. Allergy to saffron (Crocus sativus) pollen has been described in people exposed to its pollen. Saffron contains a ...
Cloning and Transcriptional Activation of the Vitamin D Receptor (Amphibians)1
VDR amphibians cloning evolution
2009/6/30
The vitamin D receptor (VDR), a member of the nuclear receptor superfamily, is an integral part of the body's calcium regulatory system. Mammalian and avian VDR genes were isolated, sequenced and char...
Cathepsin L-like cysteine proteases from Brugia malayi：cDNA cloning and comparison with Caenorhabditis elegans
Cathepsin L-like cysteine proteases Brugia malayi cDNA cloning Caenorhabditis elegans
2009/3/3
Proteases are thought to be potentially useful targets for developing medicines to control filariasis caused by parasitic nematodes. To screen cysteine proteases essential for viability of nematodes...
Cloning and characterization of an androgen-dependent acidic epididymal glycoprotein/CRISP1-like protein from the monkey
Gutknecht J Brew K Spur N. Goodfellow PN. Cloning
2009/2/18
A cDNA encoding an acidic epididymal glycoprotein (AEG)-like, CRISP1 (cysteine-rich secretory protein) protein from the monkey (Macaca mullata) epididymis has been cloned and sequenced. The monkey A...
Molecular cloning and developmental expression of the major fibrous sheath protein（FS 75）of rat sperm
Molecular cloning developmental expression major fibrous sheath protein（FS 75） rat sperm
2009/2/18
The fibrous sheath (FS) is a cytoskeletal structure that encases the axoneme in the principal piece of the spermatozoon tail. In the rat, it is composed of several proteins, of which a 75-kDa polype...
Contrin, the human homologue of a germ-cell Y-box-binding protein：cloning, expression, and chromosomal localization
human homologue germ-cell Y-box-binding protein chromosomal localization
2009/2/18
Inactivation of germ-cell-specific molecules essential for the production of functional spermatozoa could lead to attractive new means for male contraception. The mouse protein MSY2 is the mammali...
Purification, cDNA cloning, and developmental changes in the steady-state mRNA level of rat testicular tissue inhibitor of metalloproteases-2(TIMP-2)
Purification cDNA cloning steady-state mRNA level rat
2009/2/16
Using multiple high-performance liquid chromatography (HPLC) steps and high-performance electrophoresis chromatography (HPEC) in conjunction with an [125I]collagen film assay to identify inhibitors ...
Cloning and partial sequence of the rabbit androgen receptor：expression in fetal urogenital tissues
Development mesonephros androgen receptor urogenital sinus rabbit prostate
2009/2/13
Genetic studies indicate that the actions of testosterone and dihydrotestosterone are mediated by a single androgen receptor (AR). The basis for the differential effects of these hormones during d...
Cloning and Sequencing of cDNA Encoding for the Testis-Specific Fox（Vulpes Vulpes）Sperm Polypeptide Vb of the Cytochrome C Oxidase
Sperm antigen fox tissue specificity immunocontraception
2008/12/23
Identification of fox (Vulpes vulpes) sperm antigens was carried out to assess their interest as a potential target for a contraceptive vaccine. We report here the cloning and sequencing of fSP8, a ...
Molecular Cloning of Rat Spergen-3, a Spermatogenic Cell-Specific Gene-3, Encoding a Novel 75-kDa Protein Bearing EF-Hand Motifs
Spermatogenesis spermiogenesis spermatid calcium-binding protein differential display
2008/12/22
By use of differential display in combination with cDNA cloning approach, we isolated a novel rat gene designated as Spergen-3, which has an open reading frame of 2055-length nucleotides encoding ...