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Dynamin and PTP-PEST cooperatively regulate Pyk2 dephosphorylation in osteoclasts
signaling Tyrosine kinase Osteoclasts
2014/11/6
Bone loss is caused by the dysregulated activity of osteoclasts which degrade the extracellular bone matrix. The tyrosine kinase Pyk2 is highly expressed in osteoclasts, and mice lacking Pyk2 exhibit ...
Dynamin Forms a Src Kinase–sensitive Complex with Cbl and Regulates Podosomes and Osteoclast Activity
dynamin osteoclast activity
2014/11/6
Podosomes are highly dynamic actin-containing adhesion structures found in osteoclasts, macrophages, and Rous sarcoma virus (RSV)-transformed fibroblasts. After integrin engagement, Pyk2 recruits Src ...
Signaling via the Pyk2-Src-Cbl complex downstream of integrins contributes to the assembly, organization, and dynamics of podosomes, which are the transient adhesion complexes of highly motile cells s...
突触内吞关键蛋白Dynamin 的研究进展
突触 Dynamin 内吞作用
2014/11/3
囊泡循环为突触的基本生理功能,涉及到多个环节的参与,囊泡回收是整个突触囊泡循环的基础。研究表明,网格蛋白介导的内吞作用是囊泡回收的主要途径,Dynamin(发动蛋白)在此过程中发挥重要作用。Dynamin是一个分子质量约为100 kD的GTP酶,它在网格蛋白介导的内吞过程中发挥“剪刀”的作用,将网格蛋白包被小窝与质膜分离。研究发现癫痫、阿尔茨海默病、中央核肌病等多种神经性疾病与Dynamin相关。...
肠癌细胞株dynamin II的差异表达
肠癌细胞株 dynamin II 差异表达
2009/7/29
目的: 应用半定量RT-PCR方法检测dynamin II 基因在大肠侧向发育型肿瘤细胞株同SW480细胞株及 Lovo细胞株之间的差异表达.方法: 以三种肠癌细胞株为标本,分别提取总RNA,逆转录合成 cDNA,选择加入的模板量及循环数,优化PCR反应条件,确定适宜的反应体系,凝胶图像分析结果.结果: 在大肠侧向发育型肿瘤细胞株同普通隆起型肠癌细胞株之间存在着dynamin II基因的差异表达....