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Linear and nonlinear mouse sperm motility patterns
Linear nonlinear mouse sperm motility patterns
2009/2/20
A quantitative method is described for distinguishing two types of mouse sperm motility using a new parameter, the Linear Index. One hundred spermatozoa from C57BL/6-+/+ and 100 spermatozoa from C57...
Ca2+ Is Required for Mouse Sperm Capacitation and Fertilization In Vitro
capacitation acrosome reaction whiplash motility A23187 fertilization in vitro calcium
2009/2/19
The temporal involvement of Ca2+ during mouse sperm capacitation in vitro was examined by releasing sperm into Ca2+-free medium and then introducing Ca2+ (1.80 mM, final concentration) at 30 minute ...
An improved mouse sperm-oocyte plasmalemma binding assay: studies on characteristics of sperm binding in medium with or without glucose
Zona-free oocytes pronuclear zygotes in vitro fertilization
2009/2/18
Sperm binding to the oocyte plasmalemma is crucial to subsequent steps in fertilization. However, the usual in vitro assay for sperm-oocyte binding does not distinguish between nonspecific attachmen...
Evidence for the role of heterotrimeric guanine nucleotide-binding regulatory proteins in the regulation of the mouse sperm adenylyl cyclase by the egg's zona pellucida
Pertussis toxin acrosome reaction mastoparan cyclic
2009/2/18
Sperm acrosomal exocytosis is the result of a complex set of signal transduction pathways activated physiologically by the egg's extracellular matrix, the zona pellucida. In the mouse, the zona pe...
Analysis of the effect of nitric oxide synthase inhibition on mouse sperm employing a modified staining method for assessment of the acrosome reaction
Nitric oxide synthase acrosome reaction
2009/2/17
A commercially available staining kit (Spermac) was combined with swelling in a hypoosmotic medium (HOS) for simultaneous assessment of viability and acrosome reaction in mouse spermatozoa. We com...
Studies on sperm chromatin structure alterations and cytogenetic damage of mouse sperm following in vitro incubation. Studies on in vitro-incubated mouse sperm
sperm chromatin structure alterations cytogenetic damage of mouse sperm
2009/2/12
Mouse epididymal sperm incubated in Tyrode's T6 fertilization media were analyzed over time for chromosome damage by two methods. First, cytogenetic analysis was done on paternal pronuclei metapha...
The mouse sperm glycine receptor/chloride channel：cellular localization and involvement in the acrosome reaction initiated by glycine
mouse sperm glycine receptor/chloride channel cellular localization involvement glycine
2008/12/25
Previously, we reported that glycine initiates the in vitro acrosome reaction (AR) of porcine and human sperm by a mechanism that includes the glycine receptor/Cl- channel (GlyR) and that this recep...