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The relationship between the motility and morphology of spermatozoa in human semen
semen sperm motility sperm morphology male infertility
2009/2/23
High-speed videomicrography was used to assess simultaneously the morphology and motility of seminal spermatozoa from 10 fertile donors and 10 patients being evaluated for infertility. In both don...
Computer-assisted human semen analysis. Sampling errors and reproducibility
Computer-assisted human semen analysis Sampling errors reproducibility
2009/2/23
Videomicrographic computer-automated semen analysis systems allow quantitative description of sperm motility, velocity, progression, and head movement amplitude and frequency with unprecedented ease...
In vitro production of a TRH-homologous peptide and His-Pro diketopiperazine by human semen
TRH-like peptide His-Pro diketopiperazine peptide biosynthesis in semen
2009/2/23
Fresh human semen, diluted 1:1 v/v with 0.15 M NaCl- 0.05 M phosphate, pH 7.5, undergoes a 3-fold increase in total thyrotropin-releasing hormone (TRH) immunoreactivity on incubation at 4 C for 8 to...
Identification and distribution of Pz-peptidases A and B in human semen
Identification distribution Pz-peptidases A and B human semen
2009/2/23
Human semen was fractionated into fluid, particle and spermatozoal constituents using Percoll density gradient centrifugation followed by additional separation steps. All of the fractions isolated p...
Human semen contains thyrotropin releasing hormone（TRH）, a TRH-homologous peptide, and TRH-binding substances
semen TRH vasectomy exclusion ion exchange high pressure chromatography
2009/2/20
High levels of thyrotropin releasing hormone (TRH) and TRH-homologous peptide, with the general structure pGlu=X-Pro-NH2 where X is either Histidine (His) or a neutral amino acid residue, have been ...
A Method for the Determination of Chlorinated Pesticides in Human Semen
chlorinated pesticide human semen gas chromatography
2009/2/19
A method is described for the quantitative and qualitative determination of BHC and DDT isomers and HCB and Methoxychlor (DMDT) in human semen. Pesticide residues were extracted with petroleum eth...
The Sources of Carnitine in Human Semen
carnitine seminal vesicles epididymis semen vasectomy
2009/2/19
A study was initiated to determine the sources of carnitine in the male reproductive system. Free and total carnitine was measured in a group of healthy men before and after vasectomy. Each indi...
Observations on Cyclic Nucleotides in Human Semen
semen cyclic AMP cyclic GMP adenylate cyclase fructose
2009/2/19
In agreement with earlier studies, we have found that the human seminal plasma yields considerably higher values for cyclic nucleotides when compared with spermatozoa. However, we have also show...
Biochemical Aspects of the Coagulation and Liquefaction of Human Semen
semen coagulation liquefaction enzymes
2009/2/18
The coagulation and liquefaction process of human semen was studied in some detail. It was found that contact of seminal vesicle fluid with the other accessory sex gland secretions or spermatozo...
Activation of coagulation factor X in human semen
Activation coagulation factor X human semen
2009/2/17
Tissue factor (TF) is an essential cofactor for factor VII (fVII) in initiating blood coagulation. Recently, TF was shown to be present in human semen and to be associated with prostasomes that orig...
Effects of psychological stress on human semen quality
psychological stress human semen quality
2009/2/17
We investigated the relationship between psychological stress and sperm concentration, motility, and morphometry in a prospective study of 157 volunteers who were enrolled in a prepaid health plan. ...
Secretory leucocyte protease inhibitor in the male genital tract：PSA-induced proteolytic processing in human semen and tissue localization
Protease inhibitors proteolytic enzymes prostate semen seminal vesicles
2009/2/13
Secretory leucocyte protease inhibitor, SLPI, is a low-molecular-weight, acid-stable protein present in the liquid part of fresh human ejaculate but not demonstrable in the gel structure. No fragm...
The taurine and hypotaurine content of human semen
Fertility infertility oxidation antioxidant
2009/2/10
Taurine and hypotaurine levels were measured in human sperm and seminal fluid. Sperm taurine ranged from 17 nmol/mg DNA to 348 nmol/mg DNA, and hypotaurine from 0 nmol/mg DNA to 251 nmol/mg DNA. Sem...
Cryopreservation of human semen. Comparison of cryopreservatives, sources of variability, and prediction of post-thaw survival
Post-thaw sperm donor insemination infertility
2009/2/10
Human semen was cryopreserved using Human Sperm Preservation Medium, TEST-Yolk buffer, or glycerol alone. Sperm characteristics for each specimen were measured before and after freezing to determine...
Detection and quantitation of sperm-bound antibodies by flow cytometry of human semen
Spermatozoa antibody infertility
2009/2/10
Detection of sperm surface antibodies is important for the diagnosis and treatment of infertility. The authors have investigated the methodologic aspects of flow cytometry (FCM) to detect sperm-bo...