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Stereological evaluation of mouse prostate development
Stereological evaluation mouse prostate development
2009/2/18
A stereological study of the development of the mouse prostate was undertaken between the second week of postnatal life and maturity. The aim was to quantify the progressive changes in the size and ...
Evidence for the role of heterotrimeric guanine nucleotide-binding regulatory proteins in the regulation of the mouse sperm adenylyl cyclase by the egg's zona pellucida
Pertussis toxin acrosome reaction mastoparan cyclic
2009/2/18
Sperm acrosomal exocytosis is the result of a complex set of signal transduction pathways activated physiologically by the egg's extracellular matrix, the zona pellucida. In the mouse, the zona pe...
Previous studies in the rat demonstrated that lymph vessels arise from various sites in the testis and then anastomose to form the main lymph trunk toward the testicular artery. Although mice are ...
Influence of centrifugation regimes on motility, yield, and cell associations of mouse spermatozoa
Cryopreservation mechanical sensitivity damage recovery
2009/2/17
Mouse sperm are exceptionally sensitive to mechanical forces associated with pipetting and mixing. This characteristic raised the question of the sensitivity of mouse sperm to centrifugation, a st...
Round spermatids from prepubertal mouse testis can develop into normal offspring
ICSI genomic imprinting centriole
2009/2/17
We investigated fertilization and embryo development after intracytoplasmic injection of round spermatids collected from mice before puberty. The rates of normal fertilization, blastocyst formation,...
Mouse testicular sulfated glycoprotein-1：sequence analysis of the common backbone structure of prosaposins
Mouse testicular sulfated glycoprotein-1 common backbone structure prosaposins
2009/2/17
We have generated a cDNA encoding the mouse sulfated glycoprotein-1 (SGP-1) by polymerase chain reaction amplification of a mouse testicular Uni-Zap XR cDNA library with two synthetic oligonucleotid...
Distribution of mouse sulfated glycoprotein-1（prosaposin）in the testis and other tissues
Immunoperoxidase staining immunogold labeling
2009/2/17
Mouse sulfated glycoprotein-1 (SGP-1) is the homologue of rat SGP-1 and human prosaposin. Rat SGP-1 is one of the major secretory products of rat Sertoli cells in culture. Human prosaposin is the pr...
Testosterone decreases 3beta-hydroxysteroid dehydrogenase-isomerase messenger ribonucleic acid in cultured mouse Leydig cells by a strain-specific mechanism
Steroidogenesis testis inbred mice androgen
2009/2/17
We previously reported a strain-related difference in basal 3beta-hydroxysteroid dehydrogenase-isomerase (3betaHSD) activity in response to testosterone in cultured Leydig cells. The data suggested ...
The cellular mechanisms of corticotropin-releasing hormone（CRH）-stimulated steroidogenesis in mouse Leydig cells are similar to those for LH
MA-b Progesterone testosterone
2009/2/17
Previous reports have demonstrated that corticotropin-releasing hormone (CRH) treatment of primary cultures of mouse Leydig cells and MA-10 mouse Leydig tumor cells results in a dose-dependent sti...
Effects of heat stress on mouse testicular cells and sperm chromatin structure
Testicular heat stress SCSA
2009/2/17
Scrotal regions of mice were exposed to a 38.0, 40.0, or 42.0 degrees C (+/-0.1) H2O bath for 60 minutes to determine the effects of elevated temperatures on testicular cells and sperm chromatin str...
Expression of clusterin/sulfated glycoprotein-2 under conditions of heat stress in rat Sertoli cells and a mouse Sertoli cell line
SGP-2 testis HSP7O
2009/2/17
Clusterin is the major protein produced by rat Sertoli cells and is deposited onto sperm membranes; however, its function is unknown. In order to gain insight into the regulation of clusterin in Serto...
Characterization and developmental expression of a testis-specific adenosine deaminase mRNA in the mouse
testis-specific adenosine deaminase mRNA mouse
2009/2/17
Adenosine is able to alter intracellular cAMP levels and to affect the physiological functions of sperm. It also modulates FSH action through its Gi protein coupled receptors. Adenosine deaminase (A...
Analysis of the effect of nitric oxide synthase inhibition on mouse sperm employing a modified staining method for assessment of the acrosome reaction
Nitric oxide synthase acrosome reaction
2009/2/17
A commercially available staining kit (Spermac) was combined with swelling in a hypoosmotic medium (HOS) for simultaneous assessment of viability and acrosome reaction in mouse spermatozoa. We com...
Ultrastructural observations of spermatogenesis following transplantation of rat testis cells into mouse seminiferous tubules
Testis spermatogenesis transplant rat mouse
2009/2/17
The testes of busulfan-treated immunodeficient mice receiving seminiferous tubule injections of testis cells from rats were examined by light and electron microscopy. The presence of active rat sper...
Ultrastructural observations of spermatogenesis in mice resulting from transplantation of mouse spermatogonia
Testis mouse spermatogenesis electron microscopy
2009/2/16
The objective of the present study was to provide a morphological characterization of spermatogenesis following germ cell transplantation into the seminiferous tubular lumen of another mouse. The re...