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Purification of Escherichia coli-expressed HIS-tagged Maedi-Visna p25 core antigen by Ni2+-chelate affinity chromatography
recombinant antigen ELISA immunoblo
2015/1/20
In this study, recombinant histidine tagged p25 capsid protein of Maedi-Visna virus was developed. Part of the viral genome coding p25 protein was positioned downstream and in frame with a metal bindi...
Purification of L-Lysine in Simulated Moving Bed and Fixed-Bed Chromatography
ion exchange chromatography simulated moving bed purification L-lysine
2009/11/18
L-Lysine was produced by a microbial process utilizing a Corynebacterium glutamicum (ATCC 21799) strain. L-Lysine was purified from the cultivated medium by fixed-bed and simulated moving bed （SMB） c...
期刊信息
篇名
A modified procedure for fast purification of T7 RNA polymerase
语种
英文
撰写或编译
撰写
作者
Yong Li,En-duo Wang(通讯作者),Ying-lai Wang
第一作者单位
中科院上海生化所分子生物学国家重点实验室
刊物名称
Protein Expression and Purification,
...
Purification and Characterization of Pear (Pyrus communis) Polyphenol Oxidase
Purification Characterization Polyphenol Oxidase
2010/4/20
Three isoenzymes of polyphenol oxidase (PPO) were isolated from a local pear variety (Ankara Armutu) through ammonium sulfate precipitation, dialysis and gel filtration. The sample obtained from dialy...
Purification and Characterization of Glucose 6-Phosphate Dehydrogenase from Sheep Liver
Glucose 6-phosphate dehydrogenase purification sheep liver
2010/4/21
Glucose 6-phosphate dehydrogenase (D-glucose 6-phosphate: NADP+ oxidoreductase, EC 1.1.1.49; G6PD) was purified from sheep liver by a simple and rapid method. The purification process consisted of two...
Purification and Partial Characterization of Catalase from Chicken Erythrocytes and the Effect of Various Inhibitors on Enzyme Activity
Chicken erythrocyte antioxidant enzyme purification characterization isolation inhibitors
2010/4/21
Purification and Partial Characterization of Catalase from Chicken Erythrocytes and the Effect of Various Inhibitors on Enzyme Activity.
Modeling of TaqI Endonuclease Purification by HPLC
TaqI endonuclease modeling design equilibrium theory equilibrium-dispersive theory plate theory
2010/4/22
The present work is based on the previously reported purification study of TaqI endonuclease5 and aimed to simulate the experimentally obtained chromatograms in order to reduce the time and cost in th...
Partially Purification and Characterization of Polyphenol Oxidase of Quince
Quince polyphenoloxidase purification characterization
2010/4/22
Polyphenol oxidase (PPO, EC 1.14.18.1) was extracted from quince (Cydonia oblonga) by using 0.1 M phosphate buffer, pH 6.8. The polyphenol oxidase of quince was partially purified by (NH4)2SO4 and dia...
Purification and Partial Characterisation of Superoxide Dismutase from Chicken Erythrocytes
SOD purification characterisation chicken erythrocytes
2010/4/22
Superoxide dismutase (SOD), which plays a very important role in protecting organisms from oxygen toxicity, was purified from chicken erythrocyte and partially characterised. Erythrocyte membranes wer...