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Electric Field-Induced Concentration Gradients in Planar Supported Bilayers
Biophysical Phenomena Biophysics Diffusion Electricity Fluorescent Dyes Lipid Bilayers Microscopy,Fluorescence Models,Chemical
2016/5/23
A simple method of generating electric field-induced concentration gradients in planar supported bilayers has been developed. Gradients of charged, fluorescently labeled probes were visualized by epif...
A Comparison of Environmental Substrate Gradients and Calcium Selectivity in Plant Species of Calcareous Fens in Massachusetts
Calcareous fen calcium plant communities Parnassia glauca tissue analysis wetland chemistry
2014/11/17
The distribution and occurrence of plant species within a given region provides insight into the many environmental properties of that region. Although much research has been conducted on plant commun...
氯化钠密度梯度离心法制备用于显微注射的外源DNA片段Sodium Chloride Step Gradients Centrifuge to Fractionate Foreign DNA Fragment for Microinjection
显微注射 氯化钠密度梯度离心 胚胎存活率 外源基因整合率
2007/12/31
摘要DNA显微注射是生产转基因动物最可靠和最常使用的一种方法，外源DNA的纯度对显微注射的成功起着至关重要的作用。本文介绍用氯化钠密度梯度离心的方法制备用于显微注射的外源DNA片段。与传统的琼脂糖凝胶回收的方法相比较，用此方法制备的外源DNA片段对小鼠受精卵进行显微注射后，受卵体母鼠的胚胎存活率,以及子代小鼠的外源基因整合率均有明显的提高。这一方法可为进一步提高转基因动物的成功率,提供方法学上的参...