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Preliminary Study on E. coli Microbial Fuel Cell and On-electrode Taming of the Biocatalyst
microbial fuel cell E. coli taming biofilm
2009/11/13
A mediator microbial fuel cell (MFC) was constructed by using E. coli as biocatalyst and new methylene blue as electron mediator. E. coli cells were carried out in anaerobic growth prior to inoculatin...
大肠杆菌E.coli HB101(pBR322) 高密度培养过程质粒的稳定性
高密度培养 质粒稳定性 β-内酰胺酶
2009/11/12
通过正交实验考察了大肠杆菌E.coli HB101(pBR322)高密度培养过程中，不同培养条件对质粒的稳定性和β-内酰胺酶活力的影响. 结果表明，当温度在33~39℃、pH为6.4~7.2、DO为 40%~80%、 补料速率在5.4~10.8 g/h范围内变化，以及细胞密度达到27.3 g/L、比生长速率达到0.73 h-1时，质粒没有发生丢失现象，但在培养过程中β-内酰胺酶的活力降低.
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海澡酸钙胶囊化重组E.coli BL21(DE3)生产靛蓝
calcium-alginate gel gene engineering strain immobilized cells
2009/3/10
The ability of catalyzing indole into indigo of gene engineering strain expressing P450 BM3 immobilized by entrapment in calcium-alginate gel capsules was examined, and various characteristics of immo...