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旨在构建干扰胰岛素样生长因子(insulin-like growth factor-II,IGF-II)的重组质粒载体,研究IGF-II对牦牛睾丸支持细胞的影响。本研究设计并合成针对牦牛IGF-II的shRNA寡核苷酸,并将其克隆至pLKO.1质粒载体上,转染牦牛睾丸支持细胞后经嘌呤霉素筛选获得稳定的细胞株,并采用实时荧光定量PCR(qRT-PCR)和蛋白免疫印迹法检测IGF-II基因和蛋白的表达...
Short communication. An association between the G/A single nucleotide polymorphism within intron II of VIP gene and milk performance traits in dairy cattle
vasoactive intestinal peptide lactation milk utility traits
2014/10/28
Single nucleotide polymorphisms (SNPs), which are present in the encoding part of the genes responsible for important breeding functions, exert an influence on the cattle’s phenotype since their funct...
Expression Profiles of Cellular Retinol-binding Protein, Type II (CRBP II) in Erlang Mountainous Chickens
CRBP II mRNA Expression Chicken Egg Laying Rate
2016/5/16
Cellular retinol-binding protein II (CRBP II) belongs to the family of cellular retinol-binding proteins and plays a major role in absorption, transport, and metabolism of vitamin A. In addition, beca...
The Effect of Energy Supplementation on Intake and Utilisation Efficiency of Urea-treated Low-quality Roughage in Sheep II. Rumen Kinetics and Acetate Clearance Rate
Acetate Clearance Rate Glucogenic Potential Rumen Liquid Kinetics Substrate Cycle
2016/5/6
Inadequate supply of glucose or glucogenic substrates to the body tissues can affect metabolism of absorbed acetogenic metabolites from the gut and therefore, influence feed intake in ruminants. This ...
Long Term Selection for Small Body Weight in Japanese Quail II:Changes in Reproductive Traits from 60 to 65th Generations
reproductive traits long term selection small body weight genetic fitness Japanese quail
2009/7/31
Establishment of small body size poultry is an important strategy, which leads to effective management of bird-colonies and less feed consumption. And also it would be a useful experimental tool for ...
Cadmium Disrupts the Diethylstilbestrol Effect on Very-Low-Density Apolipoprotein II Gene Transcription in the Liver of Japanese Quail (Coturnix japonica)
cadmium diethylstilbestrol Japanese quail liver very-low-density apolipoprotein II
2009/7/29
To explore the possible endocrine-disrupting effect of cadmium (Cd), we have tested its role on very-low-density apolipoprotein II mRNA (apoVLDL II) expression by diethylstilbestrol (DES) in Japanese ...
VMO-II Mediates the Binding of the Chalaziferous Layer with the Vitelline Membrane in Quail Eggs
chalaziferous layer egg envelopes immunohistochemistry infundibulum quail
2009/7/29
Electron microscopic observations have revealed an electron density gradient in the chalaziferous layer of avian egg. The density is highest in the innermost sub-layer, which is bound with the vitelli...
构建豫农白猪II系繁育配套技术体系,筛选最佳杂交模式,生产种猪和商品猪。种猪生产能优良,无PSE肉。优质商品猪日增重800克以上,瘦肉率64%以上,料肉比2.9:1以上,肉质良好。建成项目示范基地2个,示范推广种猪2000头,优质商品猪40000头,实现净利润180万元。
EFFICACY OF A NOVEL BIOFILTER IN HATCHERY SANITATION: II. REMOVAL OF ODOROGENOUS POLLUTANTS
chicken hatchery odor air biofi ltration
2010/12/23
The present research assessed the treatment effi ciency of odorogenous pollutants in air from a hatchery hall vented on organic and organic-mineral beds of an enclosed-container biofi lter.
METHODS FOR CAECECTOMY OF MUSCOVY DUCKS REGARDING BALANCE EXPERIMENTS OF DIGESTIBILIY ESTABLISHING II. RECOVERY PERIOD
Muscovy ducks caecectomy recovery period
2010/12/3
Some indexes of the recovery period of Muscovy ducklings after caecectomy have been studied. It’ established, that the birds recover the water intake 9 hours, the excreting- 12-15 hours after and the ...
Utilization of Steam-treated Oil Palm Fronds in Growing Saanen Goats: II. Supplementation with Energy and Urea
Urea Fermentable Energy Microbial N Supply Oil Palm Fronds Saanen Goats
2016/4/22
The objective of this study was to evaluate the effect of protein and energy on goats fed oil palm fronds (OPF) as roughages. Twenty-four male Saanen goats aged between 7 and 8 months and weighing 23....
Nutrition Practice to Alleviate the Adverse Effects of Stress on Laying Performance, Metabolic Profile and Egg Quality in Peak Producing Hens: II. The Probiotic Supplementation
Cage Density Probiotic Laying Performance Metabolic Profile Egg Quality Peak-producing Hens
2016/4/20
In this experiment, the effects of cage density (CD) and probiotic supplementation (PS) on laying performance, metabolic profile, and egg quality in peak-producing hens were evaluated. After blocking ...
Effects of a Dietary Chitosan-Alginate-Fe(II) Complex on Meat Quality of Pig Longissimus Muscle during Ageing
Pork Quality Chitosan-Alginate-Fe(II) Complex Fatty Acids Ageing
2016/4/19
The current study was conducted to investigate the effects of dietary chitosan-alginate-Fe(II) complex (CAFC) supplementation on carcass and meat qualities of pig m. longissimus during chiller ageing....
Statistical Genetic Studies on Cattle Breeding for Dairy Productivity in Bangladesh: II. Estimation of Reciprocal and Heterosis Effects and Optimum Crossbreeding System between the Local Breeds and Exotic Breeds for Milk Performance
Bangladeshi Cattle Reciprocal Effects Heterosis Optimum Crossbreeding System
2016/4/11
Reciprocal effects among local breeds and additive and heterosis effects were estimated for total milk production per lactation (TLP), lactation length (LL) and daily milk yield (DMY). Procedures for ...