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Comparison of techniques for DNA extraction and agarose gel staining of DNA fragments using samples of Cryptosporidium
18S gene GP60 gene ethidium bromide GelRedTM
2015/4/16
Differentiating between the Cryptosporidium species and their subtypes using only microscopy is impossible. Therefore, molecular tools are indispensable for accurate species and subtype diagnosis. How...
捻转血矛线虫DNA疫苗的构建及山羊免疫保护性试验
山羊 捻转血矛线虫 H11 DNA疫苗
2008/9/17
【目的】构建捻转血矛线虫DNA疫苗，并研究该疫苗对山羊的免疫保护效果。【方法】将捻转血矛线虫重要保护性抗原H11的编码基因分H11-1、H11-2和H11-3三部分，分别亚克隆到基因疫苗载体pcDNA4/His Max C中，用酶切反应、序列测定等方法鉴定重组疫苗。将纯化的DNA疫苗肌肉注射山羊后，用RT-PCR、Western blotting和ELISA方法检测疫苗在山羊体内的转录、翻译和诱导...