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实时荧光定量PCR检测RAW264.7巨噬细胞中Th1/Th2型细胞因子转录水平方法的建立及初步应用
实时荧光定量 PCR检测RAW264.7 巨噬细胞 Th1/Th2型细胞因子 转录水平方法 初步应用
2013/9/17
建立小鼠RAW264.7巨噬细胞的Th1和Th2型细胞因子荧光定量PCR检测方法。方法 依据GenBank中小鼠的β-actin基因序列、 Th1型及Th2 型细胞因子的核苷酸序列设计特异性引物, 以RAW264.7巨噬细胞mRNA反转录后的cDNA为模板, 通过构建质粒阳性标准品, 建立检测上述细胞因子转录水平的real-time PCR方法。结果 用建立的荧光定量PCR方法, 检测牛型布鲁氏菌...
Serum Levels of Th2-Type Immunoglobulins are Increased in Weanling Mice Subjected to Acute Wasting Protein-Energy Malnutrition
Protein-energy malnutrition Serum immunoglobulins
2010/4/16
The bulk of our knowledge on immunosuppression in malnutrition comes from the experiments done on cell-mediated immunity. However, malnutrition-induced modifications of humoral immunity have been less...